钝顶螺旋藻部分原生质体及单细胞的制备与培养   总被引：21，自引：0，他引：21  

秦松  王希华  童顺  曾呈奎 《海洋与湖沼》1995,26(1):109-112

于1992年1月-1994年4月，进行超声波处理方法制备钝顶螺旋藻部分原生质体以作为基因工程受体，以及制备单细胞用于固体平板克隆化培养的研究。研究结果表明：超声波以20kHz频率、15W功率作用30s，可使藻丝体断裂成15．0±1．6个细胞长度；延长作用时间，至2－6个细胞长度时，细胞壁结构遭到破坏，形成部分原生质体；继续作用，可形成少量原生质体和大量单细胞。断裂藻丝体、部分原生质体、单细胞以及原生质体均可涂布于固体培养基上再生或生长。以一定密度涂布单细胞与原生质体，能够形成彼此分开的单个克隆，可用于筛选及遗传分析。本文提供了一种节省溶菌酶的制备螺旋藻透性体的方法，超声波作用利于外源基因的导入，而涂布培养利于进一步的筛选和形成克隆。  相似文献   

The evolution of the coral reefs in the middle and north parts of the South China Sea and its relation with tectonic and eustatic movements     

Geng Xiushan  Fu Mingzuo  Wu Baoling 《海洋学报(英文版)》1990,9(2):283-292

In this paper, the morphogenesis, stratigraphic sequences and dates of the coral reefs in the middle and north parts of the South China Sea are discussed, the position of the distributary regions of Cenozoic coral reefs in plate tectonics, the relationships of coral-reef evolutionary characteristics and dates with sea-basin spreading. Neogene sea-water transgression and Quaternary global climate-eustatic fluctuation are expounded and proved, and the latitudinal variation of the distribution of coral reefs in various geologic times are summed up.  相似文献   

A study on remote sensing models of sea ice thickness by microwave radiometry     

Zheng Quan''an  Zhang Dong  Pan Jiayi 《海洋学报(英文版)》1993,12(2):197-206

Extrapolating from the propagation theories of electromagnetic waves in a layered medium, a three-layer medium model is deduced in this paper by using microwave radiometric remote sensing technology which is suitable to first-year sea ice condition of the northern part of China seas. Comparison with in situ data indicates that for microwave wavelength of 10 cm, the coherent model gives a quite good fit result for the thickness of sea ice less than 20 cm, and the incoherent model also works well for thickness within 20 to 40 cm. Based on three theoretical models, the inversion soft ware from microwave remote sensing data for calculating the thickness of sea ice can be set up. The relative complex dielectrical constants of different types of sea ice in the Liaodong Gulf calculated by using these theoretical models and measurement data are given in this paper. The extent of their values is (0. 5-4. 0)-j(0. 07~0. 19).  相似文献   

Study on fishery biology features of spawning group of Pseudopleuronectes yokamae(Günther) in the Sheath Bay     

Chen Dagang  Liu Chang''an  Dong Guangjun 《海洋学报(英文版)》1991,10(3):471-476

Pseudopleuronectes yokamae (Günther) is one of the most important economic fish species in the genus living specially in the northwest Pacific, and is distributed in the southern area of the far east sea of Russia, Japan, Korea, the Huanghai Sea, the Bohai Sea and the northern area of the East China Sea. Usually they live in nearshore waters of these areas as geographic subpopulations. P. yokamae in the Sheath Bay belongs to a local group of the Huanghai-Bohai Sea subpopulation and is distributed in the mouth and adjacent waters of the bay all the year round, and the catch of its spawning group has important economic value in the area. In this paper the fishery biology features of spawning group of P. yokamae in the Sheath Bay is systematically studied to give biology evidence for proper utilization and the multiplification of its resources.  相似文献   

口服免疫药物后中国对虾某些血淋巴因子的测定及方法研究   总被引：53，自引：0，他引：53  

王雷  李光友  毛远兴 《海洋与湖沼》1995,26(1):34-41

于１９９１年４－９月，以海捕中国对虾亲虾及养殖中国对虾为材料，运用单向免疫扩散（ＳＲＩＤ）方法测定对虾血淋巴免疫因子，并采用经改进后的Ｈｏｒｏｗｉｔｚ等人的方法测定对虾体内的酚氧化酶（ＰＯ）活性，目的在于运用该两种方法对口服不同免疫药物的养殖对虾的血淋巴免疫因子及ＰＯ活力进行测定。对亲虾和养殖对虾口服不同免疫药物前后的测定结果表明，几种情况下的对虾血淋巴中均存在类似ＩｇＭ的因子，血细胞中存在酚氧化  相似文献   

我国海域耳鲍(Haliotis asinisne)精子发生的超微结构研究     

黄勃  邓中日  王小兵 《海洋与湖沼》2006,37(5):401-405

应用透射电子显微镜技术研究了我国海域耳鲍精子发生的全过程。结果表明,耳鲍精原细胞呈近椭圆形,染色质分布较均匀,线粒体较少;初级精母细胞较大,染色质凝聚成小块状,线粒体增多;次级精母细胞较初级精母细胞小,线粒体较多,部分线粒体发生融合,呈扁囊状。分化早期精细胞染色质凝聚成团块状,多数贴附于核膜内面,胞质中出现前顶体颗粒。分化中期精细胞染色质继续凝聚成较大的团块,线粒体在核的一端融合,形成数量少、体积大的线粒体,前顶体颗粒形成圆形外膜明显的前顶体。分化后期精核由近圆形变成长桶状,核内染色质凝聚并均质化,前顶体泡化发育成顶体,鞭毛形成,精细胞分化成精子。成熟精子为鞭毛型精子,由头部、中段和尾部(鞭毛)3部分组成。  相似文献   

闽中、闽东渔场扁舵鲣的资源生物学     

卢振彬  颜尤明  戴泉水 《台湾海峡》1992,(3)

本文以1981～1984年4～9月累计18个月在闵中、闵东渔场开展中上层鱼类资源开发性探捕调查的材料,报道了扁舵鲣的生态分布、群体结构和生活史主要特征。鱼群先后于4～7月由南而北进入本渔场,数量分布以5月为多;栖息的最适温、盐度分别为23.5～25.5℃和33～34;渔获群体由0～V龄组成;性成熟的最小年龄为Ⅰ龄,最小叉长271～280mm;生长参数L_∞=484.44mm,W_∞=2050.76g,K=0.5109,t_0=0.3598;瞬时自然死亡率为0.8937,开发比率为0.2138,属于轻度利用的鱼类资源,尚有较大的开发潜力。  相似文献   

Feeding habit and seasonal variation of stomach contents of sand dabs, Pseudopleuronectes yokohamae, in the South Bohai Sea     

Dou Shuozeng  Yang Jiming 《海洋学报(英文版)》1993,12(4):629-635

Sand dabs, an important commercial flatfish in the Bohai Sea, has recently decreased gradually in biomass, resulting from overfishing and marine pollution. Artificial culture and multiplication of sand dabs are essential and practical in China. The present paper examines the food habitof this fish to provide detailed information for its culture and multiplication.  相似文献   

好氧颗粒污泥系统快速启动试验研究   总被引：2，自引：0，他引：2  

王琳  孙磊  林跃梅 《中国海洋大学学报(自然科学版)》2006,36(2):299-302

采用3组圆柱型SBR反应器进行活性污泥颗粒化培养,接种浓度分别为12 500mg.L-1,4 400mg.L-1和1 800mg.L-1。由于采用逐步缩短沉降时间这一有效措施,系统启动20d后,颗粒污泥初步形成,污泥浓度可达到6 000~8 000mg.L-1,高于普通活性污泥系统,且沉降性能良好,SVI值在50mL.g-1左右。颗粒污泥的平均粒径稳定在0.2~0.25mm。在活性污泥颗粒化的启动试验中,在生物化学条件相同的条件下,逐步缩短沉淀时间可以加快好氧污泥颗粒化系统的启动。  相似文献   

夏季东海西部表层海水中的pCO₂及海-气界面通量   总被引：10，自引：0，他引：10  

谭燕  张龙军  王凡  胡敦欣 《海洋与湖沼》2004,35(3):239-245

根据2001年夏季长江口及东海西部海域表层海水pCO  相似文献